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pet3 h3 1 luger et  (Addgene inc)


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    Addgene inc pet3 h3 1 luger et
    Pet3 H3 1 Luger Et, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet3+h3+1+luger+et/pGL3-Basic-OPN-600+(Plasmid+%2311997)/pm41168462-674-13-11
    Average 93 stars, based on 1 article reviews
    pet3 h3 1 luger et - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Western Blot:

    Article Title: Auto-inhibition of PRC2 by the broadly expressed long isoform of AEBP2.
    Article Snippet: Reagent/resource Reference or source Identifier or catalogue number pFB1.HMBP.PrS.RBBP4 Zhang et al (2019) Addgene #125164 pFB1.HMBP.PrS.AEBP2(iso3) Zhang et al (2019) Addgene #125165 pFB1.HMBP-Prs.AEBP2iso2 This study pFB1.HMBP-Prs.AEBP2iso1 This study pFB1.HMBPPrs.AEBP2iso2.mt1A This study pFB1.HMBPPrs.AEBP2iso2.mt1K This study pFB1.HMBPPrs.AEBP2iso2.mt1Δ This study pFB1.HMBPPrs.AEBP2iso2.mt2A This study pFB1.HMBPPrs.AEBP2iso2.mt2K This study pFB1.HMBPPrs.AEBP2iso2.mt2Δ This study pFB1.HMBPPrs.AEBP2iso2.mt1A2A This study pFB1.HMBPPrs.AEBP2iso2.mt1K2K This study pFB1.HMBPPrs.AEBP2iso2.mt1Δ2Δ This study pBIG1A.HMBP.PrS.PRC2trunc This study pMB.PRC2-AEBP2LTEVJARID2 This study pMB. .. AEBP2LTEV This study pMB.MBP.JARID2 This study ATOH1_pUC18 Zhang et al (2021) Addgene #191250 pET3.H3.1 Luger et al (1997) pET3.H2A Luger et al (1997) pET3.H2B Luger et al (1997) pET3.H4 Luger et al (1997) pET3.H2A-T120C Zhang et al (2021) pWidom601.CDKN2Bp.601 Zhang et al (2019) Antibodies AEBP2 Cell Signalling Technology 14129S SUZ12 Cell Signalling Technology 3737S EPOP Active Motif 61753 MTF2 Proteintech 16208-1-AP GAPDH Proteintech 60004-1 Reagent/resource Reference or source Identifier or catalogue number JARID2 Cell Signalling Technology 13594S EZH2 (WB of tissues) Abcam Ab191250 EZH2 (WB, IP) Bracken et al, 2019 AC22 CBX7 Abcam 21873 FLAG Sigma-Aldrich F1804 TBP Cell Signalling Technology 8515 H3K27me1 Active Motif 61015 H3K27me2 (MTase) Abcam AB24684 H3K27me2 (WB) Cell Signalling Technology 9728 H3K27me3 Cell Signalling Technology 9733 H3 Abcam ab1791 IgG (ChIP) Millipore 12-370 IgG (IP) Cell Signalling Technology 2729 IgG Donkey anti-mouse IgG HRP (MTase) Jackson Immuno Research 715-035-150 IgG Goat anti-rabbit IgGHRP (MTase) (discontinued) Santa Cruz Biotechnology SANTSC-2030 IgG Goat anti-rabbit IgGHRP (MTase) Abcam AB6271 IgG Goat Anti-Rabbit (H+ L) Secondary Antibody, DyLightTM Antibody 800 4X PEG (TetO) Invitrogen SA5-35571 IgG Goat Anti-Mouse (H+ L) Secondary Antibody, DyLightTM Antibody 680 (TetO) Invitrogen 35518 Oligonucleotides and other sequence-based reagents AEBP2 CRISPR sgRNAs This study Dataset EV1 ChIP-qPCR primers This study Dataset EV1 TetO system sequences This study Dataset EV1 CpG46 DNA probe Zhang et al (2021) CpG24 DNA probe Zhang et al (2019) Chemicals, enzymes and other reagents 2i-PD0325901 Sigma-Aldrich PZ0162 2i-CHIR99021 Sigma-Aldrich SML1046 Trypsin-EDTA (0.25%) Gibco 25200056 GMEM medium Sigma-Aldrich G5154 NDiff227 medium Takara Y40002 Activin A Peprotech 120-14 bFGF Recombinant Human Protein Gibco 13256029 © The Author(s) The EMBO Journal 15 D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/resource Reference or source Identifier or catalogue number Fastqc, v0.11.9 Andrews (2010) http:// www.bioinformatics. babraham.ac.uk/projects/ fastqc/ macs2, v2.2.7.1 Feng et al (2012) https://github.com/ macs3-project/MACS/ releases/tag/v2.2.7.1 samtools, v1.9 Bonfield et al (2021) http://www.htslib.org/ bedtools, v2.27.1 Quinlan and Hall (2010) https://bedtools. readthedocs.io/en/latest/ deeptools, v3.3.0 Ramirez et al (2016) https://deeptools. readthedocs.io/en/ develop/ DESeq2, v1.22.1 Love et al (2014) https://bioconductor.org/ packages/release/bioc/ html/DESeq2.html featureCounts, v1.6.4 Liao et al (2019) https://rdocumentation. org/packages/Rsubread/ versions/1.16.1 R:pheatmap,v1.0.12 Kolde (2025) https://cran.r-project.org/ web/packages/pheatmap/ index.html R:ggplot2, v3.3.3 Wickham (2016) https://cran.r-project.org/ web/packages/ggplot2/ index.html R v3.5.1 R Core Team https://cran.r-project.org/ Prism v9.4.0 GraphPad https://www.graphpad. com/features Image quant 8.1 Cytiva Relion v3.1.2 Scheres (2012) https://relion.readthedocs. io/en/latest/Reference/ Conventions.html MotionCor2 Zheng et al (2017) CTFFIND4 Rohou and Grigorieff (2015) crYOLO Wagner et al (2019) https:// cryolo.readthedocs. io/en/stable/ CryoSPARC Punjani et al (2017) https://cryosparc.com/ Chimera Pettersen et al (2004) https://www.cgl.ucsf. edu/chimera/ COOT Emsley et al (2010) https://www2.mrc-lmb. cam.ac.uk/personal/ pemsley/coot/ PHENIX Afonine et al (2018) https://phenix-online.org/ documentation/overviews/ cryo-em_index.html MOLPROBITY Chen et al (2010) Other Cryomiller-precellys evolution touch Bertin Qubit 3.0 Fluorometer Invitrogen 2200 TapeStation System Agilent Reagent/resource Reference or source Identifier or catalogue number Licor Odyssey Fc Imager Licor ChemiDoc imaging system Bio-Rad PHERAstar plate reader BMG Labtech FLUOstar OPTIMA plate reader BMG Labtech Titan Krios electron microscope FEI K3 Summit direct electron detector Gatan Vitrobot Mark IV FEI Typhoon Trio phosphorimager Cytiva Typhoon 5 Cytiva NextSeq 500 Illumina NextSeq 500 High Output v2 kit (75 cycles) Illumina

    Chromatin Immunoprecipitation:

    Article Title: Auto-inhibition of PRC2 by the broadly expressed long isoform of AEBP2.
    Article Snippet: Reagent/resource Reference or source Identifier or catalogue number pFB1.HMBP.PrS.RBBP4 Zhang et al (2019) Addgene #125164 pFB1.HMBP.PrS.AEBP2(iso3) Zhang et al (2019) Addgene #125165 pFB1.HMBP-Prs.AEBP2iso2 This study pFB1.HMBP-Prs.AEBP2iso1 This study pFB1.HMBPPrs.AEBP2iso2.mt1A This study pFB1.HMBPPrs.AEBP2iso2.mt1K This study pFB1.HMBPPrs.AEBP2iso2.mt1Δ This study pFB1.HMBPPrs.AEBP2iso2.mt2A This study pFB1.HMBPPrs.AEBP2iso2.mt2K This study pFB1.HMBPPrs.AEBP2iso2.mt2Δ This study pFB1.HMBPPrs.AEBP2iso2.mt1A2A This study pFB1.HMBPPrs.AEBP2iso2.mt1K2K This study pFB1.HMBPPrs.AEBP2iso2.mt1Δ2Δ This study pBIG1A.HMBP.PrS.PRC2trunc This study pMB.PRC2-AEBP2LTEVJARID2 This study pMB. .. AEBP2LTEV This study pMB.MBP.JARID2 This study ATOH1_pUC18 Zhang et al (2021) Addgene #191250 pET3.H3.1 Luger et al (1997) pET3.H2A Luger et al (1997) pET3.H2B Luger et al (1997) pET3.H4 Luger et al (1997) pET3.H2A-T120C Zhang et al (2021) pWidom601.CDKN2Bp.601 Zhang et al (2019) Antibodies AEBP2 Cell Signalling Technology 14129S SUZ12 Cell Signalling Technology 3737S EPOP Active Motif 61753 MTF2 Proteintech 16208-1-AP GAPDH Proteintech 60004-1 Reagent/resource Reference or source Identifier or catalogue number JARID2 Cell Signalling Technology 13594S EZH2 (WB of tissues) Abcam Ab191250 EZH2 (WB, IP) Bracken et al, 2019 AC22 CBX7 Abcam 21873 FLAG Sigma-Aldrich F1804 TBP Cell Signalling Technology 8515 H3K27me1 Active Motif 61015 H3K27me2 (MTase) Abcam AB24684 H3K27me2 (WB) Cell Signalling Technology 9728 H3K27me3 Cell Signalling Technology 9733 H3 Abcam ab1791 IgG (ChIP) Millipore 12-370 IgG (IP) Cell Signalling Technology 2729 IgG Donkey anti-mouse IgG HRP (MTase) Jackson Immuno Research 715-035-150 IgG Goat anti-rabbit IgGHRP (MTase) (discontinued) Santa Cruz Biotechnology SANTSC-2030 IgG Goat anti-rabbit IgGHRP (MTase) Abcam AB6271 IgG Goat Anti-Rabbit (H+ L) Secondary Antibody, DyLightTM Antibody 800 4X PEG (TetO) Invitrogen SA5-35571 IgG Goat Anti-Mouse (H+ L) Secondary Antibody, DyLightTM Antibody 680 (TetO) Invitrogen 35518 Oligonucleotides and other sequence-based reagents AEBP2 CRISPR sgRNAs This study Dataset EV1 ChIP-qPCR primers This study Dataset EV1 TetO system sequences This study Dataset EV1 CpG46 DNA probe Zhang et al (2021) CpG24 DNA probe Zhang et al (2019) Chemicals, enzymes and other reagents 2i-PD0325901 Sigma-Aldrich PZ0162 2i-CHIR99021 Sigma-Aldrich SML1046 Trypsin-EDTA (0.25%) Gibco 25200056 GMEM medium Sigma-Aldrich G5154 NDiff227 medium Takara Y40002 Activin A Peprotech 120-14 bFGF Recombinant Human Protein Gibco 13256029 © The Author(s) The EMBO Journal 15 D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/resource Reference or source Identifier or catalogue number Fastqc, v0.11.9 Andrews (2010) http:// www.bioinformatics. babraham.ac.uk/projects/ fastqc/ macs2, v2.2.7.1 Feng et al (2012) https://github.com/ macs3-project/MACS/ releases/tag/v2.2.7.1 samtools, v1.9 Bonfield et al (2021) http://www.htslib.org/ bedtools, v2.27.1 Quinlan and Hall (2010) https://bedtools. readthedocs.io/en/latest/ deeptools, v3.3.0 Ramirez et al (2016) https://deeptools. readthedocs.io/en/ develop/ DESeq2, v1.22.1 Love et al (2014) https://bioconductor.org/ packages/release/bioc/ html/DESeq2.html featureCounts, v1.6.4 Liao et al (2019) https://rdocumentation. org/packages/Rsubread/ versions/1.16.1 R:pheatmap,v1.0.12 Kolde (2025) https://cran.r-project.org/ web/packages/pheatmap/ index.html R:ggplot2, v3.3.3 Wickham (2016) https://cran.r-project.org/ web/packages/ggplot2/ index.html R v3.5.1 R Core Team https://cran.r-project.org/ Prism v9.4.0 GraphPad https://www.graphpad. com/features Image quant 8.1 Cytiva Relion v3.1.2 Scheres (2012) https://relion.readthedocs. io/en/latest/Reference/ Conventions.html MotionCor2 Zheng et al (2017) CTFFIND4 Rohou and Grigorieff (2015) crYOLO Wagner et al (2019) https:// cryolo.readthedocs. io/en/stable/ CryoSPARC Punjani et al (2017) https://cryosparc.com/ Chimera Pettersen et al (2004) https://www.cgl.ucsf. edu/chimera/ COOT Emsley et al (2010) https://www2.mrc-lmb. cam.ac.uk/personal/ pemsley/coot/ PHENIX Afonine et al (2018) https://phenix-online.org/ documentation/overviews/ cryo-em_index.html MOLPROBITY Chen et al (2010) Other Cryomiller-precellys evolution touch Bertin Qubit 3.0 Fluorometer Invitrogen 2200 TapeStation System Agilent Reagent/resource Reference or source Identifier or catalogue number Licor Odyssey Fc Imager Licor ChemiDoc imaging system Bio-Rad PHERAstar plate reader BMG Labtech FLUOstar OPTIMA plate reader BMG Labtech Titan Krios electron microscope FEI K3 Summit direct electron detector Gatan Vitrobot Mark IV FEI Typhoon Trio phosphorimager Cytiva Typhoon 5 Cytiva NextSeq 500 Illumina NextSeq 500 High Output v2 kit (75 cycles) Illumina

    Sequencing:

    Article Title: Auto-inhibition of PRC2 by the broadly expressed long isoform of AEBP2.
    Article Snippet: Reagent/resource Reference or source Identifier or catalogue number pFB1.HMBP.PrS.RBBP4 Zhang et al (2019) Addgene #125164 pFB1.HMBP.PrS.AEBP2(iso3) Zhang et al (2019) Addgene #125165 pFB1.HMBP-Prs.AEBP2iso2 This study pFB1.HMBP-Prs.AEBP2iso1 This study pFB1.HMBPPrs.AEBP2iso2.mt1A This study pFB1.HMBPPrs.AEBP2iso2.mt1K This study pFB1.HMBPPrs.AEBP2iso2.mt1Δ This study pFB1.HMBPPrs.AEBP2iso2.mt2A This study pFB1.HMBPPrs.AEBP2iso2.mt2K This study pFB1.HMBPPrs.AEBP2iso2.mt2Δ This study pFB1.HMBPPrs.AEBP2iso2.mt1A2A This study pFB1.HMBPPrs.AEBP2iso2.mt1K2K This study pFB1.HMBPPrs.AEBP2iso2.mt1Δ2Δ This study pBIG1A.HMBP.PrS.PRC2trunc This study pMB.PRC2-AEBP2LTEVJARID2 This study pMB. .. AEBP2LTEV This study pMB.MBP.JARID2 This study ATOH1_pUC18 Zhang et al (2021) Addgene #191250 pET3.H3.1 Luger et al (1997) pET3.H2A Luger et al (1997) pET3.H2B Luger et al (1997) pET3.H4 Luger et al (1997) pET3.H2A-T120C Zhang et al (2021) pWidom601.CDKN2Bp.601 Zhang et al (2019) Antibodies AEBP2 Cell Signalling Technology 14129S SUZ12 Cell Signalling Technology 3737S EPOP Active Motif 61753 MTF2 Proteintech 16208-1-AP GAPDH Proteintech 60004-1 Reagent/resource Reference or source Identifier or catalogue number JARID2 Cell Signalling Technology 13594S EZH2 (WB of tissues) Abcam Ab191250 EZH2 (WB, IP) Bracken et al, 2019 AC22 CBX7 Abcam 21873 FLAG Sigma-Aldrich F1804 TBP Cell Signalling Technology 8515 H3K27me1 Active Motif 61015 H3K27me2 (MTase) Abcam AB24684 H3K27me2 (WB) Cell Signalling Technology 9728 H3K27me3 Cell Signalling Technology 9733 H3 Abcam ab1791 IgG (ChIP) Millipore 12-370 IgG (IP) Cell Signalling Technology 2729 IgG Donkey anti-mouse IgG HRP (MTase) Jackson Immuno Research 715-035-150 IgG Goat anti-rabbit IgGHRP (MTase) (discontinued) Santa Cruz Biotechnology SANTSC-2030 IgG Goat anti-rabbit IgGHRP (MTase) Abcam AB6271 IgG Goat Anti-Rabbit (H+ L) Secondary Antibody, DyLightTM Antibody 800 4X PEG (TetO) Invitrogen SA5-35571 IgG Goat Anti-Mouse (H+ L) Secondary Antibody, DyLightTM Antibody 680 (TetO) Invitrogen 35518 Oligonucleotides and other sequence-based reagents AEBP2 CRISPR sgRNAs This study Dataset EV1 ChIP-qPCR primers This study Dataset EV1 TetO system sequences This study Dataset EV1 CpG46 DNA probe Zhang et al (2021) CpG24 DNA probe Zhang et al (2019) Chemicals, enzymes and other reagents 2i-PD0325901 Sigma-Aldrich PZ0162 2i-CHIR99021 Sigma-Aldrich SML1046 Trypsin-EDTA (0.25%) Gibco 25200056 GMEM medium Sigma-Aldrich G5154 NDiff227 medium Takara Y40002 Activin A Peprotech 120-14 bFGF Recombinant Human Protein Gibco 13256029 © The Author(s) The EMBO Journal 15 D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/resource Reference or source Identifier or catalogue number Fastqc, v0.11.9 Andrews (2010) http:// www.bioinformatics. babraham.ac.uk/projects/ fastqc/ macs2, v2.2.7.1 Feng et al (2012) https://github.com/ macs3-project/MACS/ releases/tag/v2.2.7.1 samtools, v1.9 Bonfield et al (2021) http://www.htslib.org/ bedtools, v2.27.1 Quinlan and Hall (2010) https://bedtools. readthedocs.io/en/latest/ deeptools, v3.3.0 Ramirez et al (2016) https://deeptools. readthedocs.io/en/ develop/ DESeq2, v1.22.1 Love et al (2014) https://bioconductor.org/ packages/release/bioc/ html/DESeq2.html featureCounts, v1.6.4 Liao et al (2019) https://rdocumentation. org/packages/Rsubread/ versions/1.16.1 R:pheatmap,v1.0.12 Kolde (2025) https://cran.r-project.org/ web/packages/pheatmap/ index.html R:ggplot2, v3.3.3 Wickham (2016) https://cran.r-project.org/ web/packages/ggplot2/ index.html R v3.5.1 R Core Team https://cran.r-project.org/ Prism v9.4.0 GraphPad https://www.graphpad. com/features Image quant 8.1 Cytiva Relion v3.1.2 Scheres (2012) https://relion.readthedocs. io/en/latest/Reference/ Conventions.html MotionCor2 Zheng et al (2017) CTFFIND4 Rohou and Grigorieff (2015) crYOLO Wagner et al (2019) https:// cryolo.readthedocs. io/en/stable/ CryoSPARC Punjani et al (2017) https://cryosparc.com/ Chimera Pettersen et al (2004) https://www.cgl.ucsf. edu/chimera/ COOT Emsley et al (2010) https://www2.mrc-lmb. cam.ac.uk/personal/ pemsley/coot/ PHENIX Afonine et al (2018) https://phenix-online.org/ documentation/overviews/ cryo-em_index.html MOLPROBITY Chen et al (2010) Other Cryomiller-precellys evolution touch Bertin Qubit 3.0 Fluorometer Invitrogen 2200 TapeStation System Agilent Reagent/resource Reference or source Identifier or catalogue number Licor Odyssey Fc Imager Licor ChemiDoc imaging system Bio-Rad PHERAstar plate reader BMG Labtech FLUOstar OPTIMA plate reader BMG Labtech Titan Krios electron microscope FEI K3 Summit direct electron detector Gatan Vitrobot Mark IV FEI Typhoon Trio phosphorimager Cytiva Typhoon 5 Cytiva NextSeq 500 Illumina NextSeq 500 High Output v2 kit (75 cycles) Illumina

    CRISPR:

    Article Title: Auto-inhibition of PRC2 by the broadly expressed long isoform of AEBP2.
    Article Snippet: Reagent/resource Reference or source Identifier or catalogue number pFB1.HMBP.PrS.RBBP4 Zhang et al (2019) Addgene #125164 pFB1.HMBP.PrS.AEBP2(iso3) Zhang et al (2019) Addgene #125165 pFB1.HMBP-Prs.AEBP2iso2 This study pFB1.HMBP-Prs.AEBP2iso1 This study pFB1.HMBPPrs.AEBP2iso2.mt1A This study pFB1.HMBPPrs.AEBP2iso2.mt1K This study pFB1.HMBPPrs.AEBP2iso2.mt1Δ This study pFB1.HMBPPrs.AEBP2iso2.mt2A This study pFB1.HMBPPrs.AEBP2iso2.mt2K This study pFB1.HMBPPrs.AEBP2iso2.mt2Δ This study pFB1.HMBPPrs.AEBP2iso2.mt1A2A This study pFB1.HMBPPrs.AEBP2iso2.mt1K2K This study pFB1.HMBPPrs.AEBP2iso2.mt1Δ2Δ This study pBIG1A.HMBP.PrS.PRC2trunc This study pMB.PRC2-AEBP2LTEVJARID2 This study pMB. .. AEBP2LTEV This study pMB.MBP.JARID2 This study ATOH1_pUC18 Zhang et al (2021) Addgene #191250 pET3.H3.1 Luger et al (1997) pET3.H2A Luger et al (1997) pET3.H2B Luger et al (1997) pET3.H4 Luger et al (1997) pET3.H2A-T120C Zhang et al (2021) pWidom601.CDKN2Bp.601 Zhang et al (2019) Antibodies AEBP2 Cell Signalling Technology 14129S SUZ12 Cell Signalling Technology 3737S EPOP Active Motif 61753 MTF2 Proteintech 16208-1-AP GAPDH Proteintech 60004-1 Reagent/resource Reference or source Identifier or catalogue number JARID2 Cell Signalling Technology 13594S EZH2 (WB of tissues) Abcam Ab191250 EZH2 (WB, IP) Bracken et al, 2019 AC22 CBX7 Abcam 21873 FLAG Sigma-Aldrich F1804 TBP Cell Signalling Technology 8515 H3K27me1 Active Motif 61015 H3K27me2 (MTase) Abcam AB24684 H3K27me2 (WB) Cell Signalling Technology 9728 H3K27me3 Cell Signalling Technology 9733 H3 Abcam ab1791 IgG (ChIP) Millipore 12-370 IgG (IP) Cell Signalling Technology 2729 IgG Donkey anti-mouse IgG HRP (MTase) Jackson Immuno Research 715-035-150 IgG Goat anti-rabbit IgGHRP (MTase) (discontinued) Santa Cruz Biotechnology SANTSC-2030 IgG Goat anti-rabbit IgGHRP (MTase) Abcam AB6271 IgG Goat Anti-Rabbit (H+ L) Secondary Antibody, DyLightTM Antibody 800 4X PEG (TetO) Invitrogen SA5-35571 IgG Goat Anti-Mouse (H+ L) Secondary Antibody, DyLightTM Antibody 680 (TetO) Invitrogen 35518 Oligonucleotides and other sequence-based reagents AEBP2 CRISPR sgRNAs This study Dataset EV1 ChIP-qPCR primers This study Dataset EV1 TetO system sequences This study Dataset EV1 CpG46 DNA probe Zhang et al (2021) CpG24 DNA probe Zhang et al (2019) Chemicals, enzymes and other reagents 2i-PD0325901 Sigma-Aldrich PZ0162 2i-CHIR99021 Sigma-Aldrich SML1046 Trypsin-EDTA (0.25%) Gibco 25200056 GMEM medium Sigma-Aldrich G5154 NDiff227 medium Takara Y40002 Activin A Peprotech 120-14 bFGF Recombinant Human Protein Gibco 13256029 © The Author(s) The EMBO Journal 15 D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/resource Reference or source Identifier or catalogue number Fastqc, v0.11.9 Andrews (2010) http:// www.bioinformatics. babraham.ac.uk/projects/ fastqc/ macs2, v2.2.7.1 Feng et al (2012) https://github.com/ macs3-project/MACS/ releases/tag/v2.2.7.1 samtools, v1.9 Bonfield et al (2021) http://www.htslib.org/ bedtools, v2.27.1 Quinlan and Hall (2010) https://bedtools. readthedocs.io/en/latest/ deeptools, v3.3.0 Ramirez et al (2016) https://deeptools. readthedocs.io/en/ develop/ DESeq2, v1.22.1 Love et al (2014) https://bioconductor.org/ packages/release/bioc/ html/DESeq2.html featureCounts, v1.6.4 Liao et al (2019) https://rdocumentation. org/packages/Rsubread/ versions/1.16.1 R:pheatmap,v1.0.12 Kolde (2025) https://cran.r-project.org/ web/packages/pheatmap/ index.html R:ggplot2, v3.3.3 Wickham (2016) https://cran.r-project.org/ web/packages/ggplot2/ index.html R v3.5.1 R Core Team https://cran.r-project.org/ Prism v9.4.0 GraphPad https://www.graphpad. com/features Image quant 8.1 Cytiva Relion v3.1.2 Scheres (2012) https://relion.readthedocs. io/en/latest/Reference/ Conventions.html MotionCor2 Zheng et al (2017) CTFFIND4 Rohou and Grigorieff (2015) crYOLO Wagner et al (2019) https:// cryolo.readthedocs. io/en/stable/ CryoSPARC Punjani et al (2017) https://cryosparc.com/ Chimera Pettersen et al (2004) https://www.cgl.ucsf. edu/chimera/ COOT Emsley et al (2010) https://www2.mrc-lmb. cam.ac.uk/personal/ pemsley/coot/ PHENIX Afonine et al (2018) https://phenix-online.org/ documentation/overviews/ cryo-em_index.html MOLPROBITY Chen et al (2010) Other Cryomiller-precellys evolution touch Bertin Qubit 3.0 Fluorometer Invitrogen 2200 TapeStation System Agilent Reagent/resource Reference or source Identifier or catalogue number Licor Odyssey Fc Imager Licor ChemiDoc imaging system Bio-Rad PHERAstar plate reader BMG Labtech FLUOstar OPTIMA plate reader BMG Labtech Titan Krios electron microscope FEI K3 Summit direct electron detector Gatan Vitrobot Mark IV FEI Typhoon Trio phosphorimager Cytiva Typhoon 5 Cytiva NextSeq 500 Illumina NextSeq 500 High Output v2 kit (75 cycles) Illumina

    ChIP-qPCR:

    Article Title: Auto-inhibition of PRC2 by the broadly expressed long isoform of AEBP2.
    Article Snippet: Reagent/resource Reference or source Identifier or catalogue number pFB1.HMBP.PrS.RBBP4 Zhang et al (2019) Addgene #125164 pFB1.HMBP.PrS.AEBP2(iso3) Zhang et al (2019) Addgene #125165 pFB1.HMBP-Prs.AEBP2iso2 This study pFB1.HMBP-Prs.AEBP2iso1 This study pFB1.HMBPPrs.AEBP2iso2.mt1A This study pFB1.HMBPPrs.AEBP2iso2.mt1K This study pFB1.HMBPPrs.AEBP2iso2.mt1Δ This study pFB1.HMBPPrs.AEBP2iso2.mt2A This study pFB1.HMBPPrs.AEBP2iso2.mt2K This study pFB1.HMBPPrs.AEBP2iso2.mt2Δ This study pFB1.HMBPPrs.AEBP2iso2.mt1A2A This study pFB1.HMBPPrs.AEBP2iso2.mt1K2K This study pFB1.HMBPPrs.AEBP2iso2.mt1Δ2Δ This study pBIG1A.HMBP.PrS.PRC2trunc This study pMB.PRC2-AEBP2LTEVJARID2 This study pMB. .. AEBP2LTEV This study pMB.MBP.JARID2 This study ATOH1_pUC18 Zhang et al (2021) Addgene #191250 pET3.H3.1 Luger et al (1997) pET3.H2A Luger et al (1997) pET3.H2B Luger et al (1997) pET3.H4 Luger et al (1997) pET3.H2A-T120C Zhang et al (2021) pWidom601.CDKN2Bp.601 Zhang et al (2019) Antibodies AEBP2 Cell Signalling Technology 14129S SUZ12 Cell Signalling Technology 3737S EPOP Active Motif 61753 MTF2 Proteintech 16208-1-AP GAPDH Proteintech 60004-1 Reagent/resource Reference or source Identifier or catalogue number JARID2 Cell Signalling Technology 13594S EZH2 (WB of tissues) Abcam Ab191250 EZH2 (WB, IP) Bracken et al, 2019 AC22 CBX7 Abcam 21873 FLAG Sigma-Aldrich F1804 TBP Cell Signalling Technology 8515 H3K27me1 Active Motif 61015 H3K27me2 (MTase) Abcam AB24684 H3K27me2 (WB) Cell Signalling Technology 9728 H3K27me3 Cell Signalling Technology 9733 H3 Abcam ab1791 IgG (ChIP) Millipore 12-370 IgG (IP) Cell Signalling Technology 2729 IgG Donkey anti-mouse IgG HRP (MTase) Jackson Immuno Research 715-035-150 IgG Goat anti-rabbit IgGHRP (MTase) (discontinued) Santa Cruz Biotechnology SANTSC-2030 IgG Goat anti-rabbit IgGHRP (MTase) Abcam AB6271 IgG Goat Anti-Rabbit (H+ L) Secondary Antibody, DyLightTM Antibody 800 4X PEG (TetO) Invitrogen SA5-35571 IgG Goat Anti-Mouse (H+ L) Secondary Antibody, DyLightTM Antibody 680 (TetO) Invitrogen 35518 Oligonucleotides and other sequence-based reagents AEBP2 CRISPR sgRNAs This study Dataset EV1 ChIP-qPCR primers This study Dataset EV1 TetO system sequences This study Dataset EV1 CpG46 DNA probe Zhang et al (2021) CpG24 DNA probe Zhang et al (2019) Chemicals, enzymes and other reagents 2i-PD0325901 Sigma-Aldrich PZ0162 2i-CHIR99021 Sigma-Aldrich SML1046 Trypsin-EDTA (0.25%) Gibco 25200056 GMEM medium Sigma-Aldrich G5154 NDiff227 medium Takara Y40002 Activin A Peprotech 120-14 bFGF Recombinant Human Protein Gibco 13256029 © The Author(s) The EMBO Journal 15 D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/resource Reference or source Identifier or catalogue number Fastqc, v0.11.9 Andrews (2010) http:// www.bioinformatics. babraham.ac.uk/projects/ fastqc/ macs2, v2.2.7.1 Feng et al (2012) https://github.com/ macs3-project/MACS/ releases/tag/v2.2.7.1 samtools, v1.9 Bonfield et al (2021) http://www.htslib.org/ bedtools, v2.27.1 Quinlan and Hall (2010) https://bedtools. readthedocs.io/en/latest/ deeptools, v3.3.0 Ramirez et al (2016) https://deeptools. readthedocs.io/en/ develop/ DESeq2, v1.22.1 Love et al (2014) https://bioconductor.org/ packages/release/bioc/ html/DESeq2.html featureCounts, v1.6.4 Liao et al (2019) https://rdocumentation. org/packages/Rsubread/ versions/1.16.1 R:pheatmap,v1.0.12 Kolde (2025) https://cran.r-project.org/ web/packages/pheatmap/ index.html R:ggplot2, v3.3.3 Wickham (2016) https://cran.r-project.org/ web/packages/ggplot2/ index.html R v3.5.1 R Core Team https://cran.r-project.org/ Prism v9.4.0 GraphPad https://www.graphpad. com/features Image quant 8.1 Cytiva Relion v3.1.2 Scheres (2012) https://relion.readthedocs. io/en/latest/Reference/ Conventions.html MotionCor2 Zheng et al (2017) CTFFIND4 Rohou and Grigorieff (2015) crYOLO Wagner et al (2019) https:// cryolo.readthedocs. io/en/stable/ CryoSPARC Punjani et al (2017) https://cryosparc.com/ Chimera Pettersen et al (2004) https://www.cgl.ucsf. edu/chimera/ COOT Emsley et al (2010) https://www2.mrc-lmb. cam.ac.uk/personal/ pemsley/coot/ PHENIX Afonine et al (2018) https://phenix-online.org/ documentation/overviews/ cryo-em_index.html MOLPROBITY Chen et al (2010) Other Cryomiller-precellys evolution touch Bertin Qubit 3.0 Fluorometer Invitrogen 2200 TapeStation System Agilent Reagent/resource Reference or source Identifier or catalogue number Licor Odyssey Fc Imager Licor ChemiDoc imaging system Bio-Rad PHERAstar plate reader BMG Labtech FLUOstar OPTIMA plate reader BMG Labtech Titan Krios electron microscope FEI K3 Summit direct electron detector Gatan Vitrobot Mark IV FEI Typhoon Trio phosphorimager Cytiva Typhoon 5 Cytiva NextSeq 500 Illumina NextSeq 500 High Output v2 kit (75 cycles) Illumina

    Recombinant:

    Article Title: Auto-inhibition of PRC2 by the broadly expressed long isoform of AEBP2.
    Article Snippet: Reagent/resource Reference or source Identifier or catalogue number pFB1.HMBP.PrS.RBBP4 Zhang et al (2019) Addgene #125164 pFB1.HMBP.PrS.AEBP2(iso3) Zhang et al (2019) Addgene #125165 pFB1.HMBP-Prs.AEBP2iso2 This study pFB1.HMBP-Prs.AEBP2iso1 This study pFB1.HMBPPrs.AEBP2iso2.mt1A This study pFB1.HMBPPrs.AEBP2iso2.mt1K This study pFB1.HMBPPrs.AEBP2iso2.mt1Δ This study pFB1.HMBPPrs.AEBP2iso2.mt2A This study pFB1.HMBPPrs.AEBP2iso2.mt2K This study pFB1.HMBPPrs.AEBP2iso2.mt2Δ This study pFB1.HMBPPrs.AEBP2iso2.mt1A2A This study pFB1.HMBPPrs.AEBP2iso2.mt1K2K This study pFB1.HMBPPrs.AEBP2iso2.mt1Δ2Δ This study pBIG1A.HMBP.PrS.PRC2trunc This study pMB.PRC2-AEBP2LTEVJARID2 This study pMB. .. AEBP2LTEV This study pMB.MBP.JARID2 This study ATOH1_pUC18 Zhang et al (2021) Addgene #191250 pET3.H3.1 Luger et al (1997) pET3.H2A Luger et al (1997) pET3.H2B Luger et al (1997) pET3.H4 Luger et al (1997) pET3.H2A-T120C Zhang et al (2021) pWidom601.CDKN2Bp.601 Zhang et al (2019) Antibodies AEBP2 Cell Signalling Technology 14129S SUZ12 Cell Signalling Technology 3737S EPOP Active Motif 61753 MTF2 Proteintech 16208-1-AP GAPDH Proteintech 60004-1 Reagent/resource Reference or source Identifier or catalogue number JARID2 Cell Signalling Technology 13594S EZH2 (WB of tissues) Abcam Ab191250 EZH2 (WB, IP) Bracken et al, 2019 AC22 CBX7 Abcam 21873 FLAG Sigma-Aldrich F1804 TBP Cell Signalling Technology 8515 H3K27me1 Active Motif 61015 H3K27me2 (MTase) Abcam AB24684 H3K27me2 (WB) Cell Signalling Technology 9728 H3K27me3 Cell Signalling Technology 9733 H3 Abcam ab1791 IgG (ChIP) Millipore 12-370 IgG (IP) Cell Signalling Technology 2729 IgG Donkey anti-mouse IgG HRP (MTase) Jackson Immuno Research 715-035-150 IgG Goat anti-rabbit IgGHRP (MTase) (discontinued) Santa Cruz Biotechnology SANTSC-2030 IgG Goat anti-rabbit IgGHRP (MTase) Abcam AB6271 IgG Goat Anti-Rabbit (H+ L) Secondary Antibody, DyLightTM Antibody 800 4X PEG (TetO) Invitrogen SA5-35571 IgG Goat Anti-Mouse (H+ L) Secondary Antibody, DyLightTM Antibody 680 (TetO) Invitrogen 35518 Oligonucleotides and other sequence-based reagents AEBP2 CRISPR sgRNAs This study Dataset EV1 ChIP-qPCR primers This study Dataset EV1 TetO system sequences This study Dataset EV1 CpG46 DNA probe Zhang et al (2021) CpG24 DNA probe Zhang et al (2019) Chemicals, enzymes and other reagents 2i-PD0325901 Sigma-Aldrich PZ0162 2i-CHIR99021 Sigma-Aldrich SML1046 Trypsin-EDTA (0.25%) Gibco 25200056 GMEM medium Sigma-Aldrich G5154 NDiff227 medium Takara Y40002 Activin A Peprotech 120-14 bFGF Recombinant Human Protein Gibco 13256029 © The Author(s) The EMBO Journal 15 D ow nloaded from https://w w w .em bopress.org on N ovem ber 8, 2025 from IP 2400:1a00:4ba5:c093:f5ff:4dcd:7589:c171. .. Reagent/resource Reference or source Identifier or catalogue number Fastqc, v0.11.9 Andrews (2010) http:// www.bioinformatics. babraham.ac.uk/projects/ fastqc/ macs2, v2.2.7.1 Feng et al (2012) https://github.com/ macs3-project/MACS/ releases/tag/v2.2.7.1 samtools, v1.9 Bonfield et al (2021) http://www.htslib.org/ bedtools, v2.27.1 Quinlan and Hall (2010) https://bedtools. readthedocs.io/en/latest/ deeptools, v3.3.0 Ramirez et al (2016) https://deeptools. readthedocs.io/en/ develop/ DESeq2, v1.22.1 Love et al (2014) https://bioconductor.org/ packages/release/bioc/ html/DESeq2.html featureCounts, v1.6.4 Liao et al (2019) https://rdocumentation. org/packages/Rsubread/ versions/1.16.1 R:pheatmap,v1.0.12 Kolde (2025) https://cran.r-project.org/ web/packages/pheatmap/ index.html R:ggplot2, v3.3.3 Wickham (2016) https://cran.r-project.org/ web/packages/ggplot2/ index.html R v3.5.1 R Core Team https://cran.r-project.org/ Prism v9.4.0 GraphPad https://www.graphpad. com/features Image quant 8.1 Cytiva Relion v3.1.2 Scheres (2012) https://relion.readthedocs. io/en/latest/Reference/ Conventions.html MotionCor2 Zheng et al (2017) CTFFIND4 Rohou and Grigorieff (2015) crYOLO Wagner et al (2019) https:// cryolo.readthedocs. io/en/stable/ CryoSPARC Punjani et al (2017) https://cryosparc.com/ Chimera Pettersen et al (2004) https://www.cgl.ucsf. edu/chimera/ COOT Emsley et al (2010) https://www2.mrc-lmb. cam.ac.uk/personal/ pemsley/coot/ PHENIX Afonine et al (2018) https://phenix-online.org/ documentation/overviews/ cryo-em_index.html MOLPROBITY Chen et al (2010) Other Cryomiller-precellys evolution touch Bertin Qubit 3.0 Fluorometer Invitrogen 2200 TapeStation System Agilent Reagent/resource Reference or source Identifier or catalogue number Licor Odyssey Fc Imager Licor ChemiDoc imaging system Bio-Rad PHERAstar plate reader BMG Labtech FLUOstar OPTIMA plate reader BMG Labtech Titan Krios electron microscope FEI K3 Summit direct electron detector Gatan Vitrobot Mark IV FEI Typhoon Trio phosphorimager Cytiva Typhoon 5 Cytiva NextSeq 500 Illumina NextSeq 500 High Output v2 kit (75 cycles) Illumina



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